Interakce iontů kovů s imobilizovanou DNázou I
| Title in English | Interactions of metal ions with immobilized DNase I |
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| Authors | |
| Year of publication | 2002 |
| Type | Article in Proceedings |
| Conference | VI. Pracovní setkání biochemiků a molekulárních biologů |
| MU Faculty or unit | |
| Citation | |
| Field | Microbiology, virology |
| Keywords | DNA cleavage; DNase I; immobilization; activating ions; lanthanides |
| Description | DNaseI cleaves both strands od DNA molecule by chance in the presence of Mg2+ and Ca2+ ions. DNaseI immobilised on magnetic cellulose was choosed as a model system. Lanthanides have the same chemical properties as Ca2+ ions. Lanthanides have an inhibitory effect on enzyme activity activated with divalent cations in many cases.Cleavage of chromosomal DNA was not detected using DNaseI activated with La3+ ions. Cleavage of plasmid DNA was detected in the presence of some lanthanides ions only. |
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