Degradation of 4-nitrocatechol and other nitroaromatic compounds by Rhodococcus opacus J3

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Authors

NAVRÁTILOVÁ Jitka KOTOUČKOVÁ Ludmila NĚMEC Miroslav SEDLÁČEK Ivo DURNOVÁ Eva

Year of publication 2001
Type Article in Proceedings
Conference V. Pracovní setkání biochemiků a molekulárních biologů
MU Faculty or unit

Faculty of Science

Citation
Field Microbiology, virology
Keywords 4-nitrocatechol; Rhodococcus opacus
Description Rhodococcus opacus J3 is capable to degrade nitroaromatic compounds.This strain was isolated from soil by selectively enrichment with 4-nitrocatechol in SBM medium. Rhodococcus opacus J3 is gram-positive, strict aerobic bacterium who is typical by growth rod-coccus cycle.Degradation of 9 nitroaromatics were tested.We confirmed degradation of 5 substrates:4-nitrocatechol, 4-nitroguaiacol, 5-nitroguaiacol, 3-nitrophenol and 2,4-dinitrobenzoic acid. Concentration of nitroaromatics and nitrites released were measured spectrophotometricaly. The process of degradation was monitored on automatic microbiological system Bioscreen.
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