Application of Capillary Zone Electrophoresis to Study the Properties of Rhodanese from Acidithiobacillus ferrooxidans
| Authors | |
|---|---|
| Year of publication | 2001 |
| Type | Article in Periodical |
| Magazine / Source | Folia Microbiologica |
| MU Faculty or unit | |
| Citation | |
| Field | Biochemistry |
| Keywords | rhodanese; CZE |
| Description | The new capillary zone electrophoretic method was applied for the assay of the enzymatic activity of the rhodanese from Acidithiobacillus ferrooxidans. The enzyme activities determined by capillary zone electrophoresis were compared with those determined by discontinuous spectrophotometric method. The values obtained were in a good agreement. The method was also used to evaluate Michaelis constants for both substrates; a new approach was developed to solve the problem with variable ionic strength of the samples. The other properties of the enzyme such as the pH and temperature optima were determined as well. |
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