Developmental Consequences of ipt Activation in Germinating Tobacco Seedlings
| Authors | |
|---|---|
| Year of publication | 2001 |
| Type | Article in Proceedings |
| Conference | 17th International Conference on Plant Growth Substances, Brno, Czech Republic, July 1-6, 2001, Abstracts |
| MU Faculty or unit | |
| Citation | |
| Field | Genetics and molecular biology |
| Keywords | transcription activation system; isopentenyl transferase; cytokinins; tobacco; Arabidopsis |
| Description | A transcription activation system based on a synthetic promoter pOp and a corresponding chimeric transcription activator LhG4 was employed to achieve regulatable expression of isopentenyl transferase, IPT, in transgenic tobacco. The system allowed us to analyze in detail developmental consequences of ipt activation in germination and early seedling development. Here we present analysis of (i) dynamics of changes in cytokinin metabolism upon ipt activation and (ii) subsequent chain of developmental alterations leading to transition from originally almost normal seedling to a completely distinct teratoma structure. Major differences in tobacco and Arabidopsis response to ipt activation during germination will be discussed. |
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