Stanovení metallothioneinu v tkáních okouna říčního a kapra obecného(Perca fluviatilis L., Cyprinus carpio L.)

Knápek,  Jan; Konečná,  Marie; Jarkovský,  Jiří; Trnková,  Libuše; Kizek,  René

Stanovení metallothioneinu v tkáních okouna říčního a kapra obecného(Perca fluviatilis L., Cyprinus carpio L.)

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Title in English	Detection of metallothionein in fish tissues-Perca fluviatilis L., Cyprinus carpio L.
Authors	KNÁPEK Jan KONEČNÁ Marie JARKOVSKÝ Jiří TRNKOVÁ Libuše KIZEK René
Year of publication	2001
Type	Article in Proceedings
Conference	Sborník:5.pracovní setkání biochemiků a molekulárních biologů, Brno 2001
MU Faculty or unit	Faculty of Science
Citation
Field	Electrochemistry
Keywords	Perca fluviatilis L.; Cyprinus carpio L.; metallothionein; Chronopotentiometric stripping analysis; catalytic process in hydrogen evolution
Description	Metalloproteins are low molecular proteins with catalytic and homeostatic function. The aim of this work was the quantification of metallothionein (MT)which produces at HMDE a well developed catalytic signal(peak H). The highest sensitivity of the MT determination was obtained with CPSA. Due to catalytic hydrogen evolution at highly negative potentials, picomolar concentrations of MT in various tissues were found.
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