Application of Elimination Methods in Electrochemical Study of DNA

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Authors

TRNKOVÁ Libuše KIZEK René DRAČKA Oldřich

Year of publication 2000
Type Article in Periodical
Magazine / Source Journal of Biomolecular Structure and Dynamics
MU Faculty or unit

Faculty of Science

Citation
Field Electrochemistry
Keywords Elimination of particular currents; elimination polarography/voltammetry; DNA; adsorbed species
Description Adsorptive stripping voltammetry has been extended by elimination voltammetry with the linear scan rate (EVLS). EVLS offers the elimination of selected currents from the linear scan voltammetry results. It can be achieved by an elimination function formed by a linear combination of total currents measured at different scan rates. The problem of substance adsorption influence on the elimination voltammetry results has been studied. Theoretical curves of an irreversible current with adsorbed substance being electroactive were calculated for three elimination functions. As a practical application, the adsorptive stripping voltammetry of thermally denatured DNA on a hanging mercury electrode has been developed. While the LSV signal of single-stranded DNA at low concentrations gives a slight hint of the cathodic peak, the EVLS provides quite a clear signal in the form of the peak-counterpeak. It is possible to determinate DNA at concentrations below of micrograms per milliliter.
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