Application of Elimination Methods in Electrochemical Study of DNA
| Authors | |
|---|---|
| Year of publication | 2000 |
| Type | Article in Periodical |
| Magazine / Source | Journal of Biomolecular Structure and Dynamics |
| MU Faculty or unit | |
| Citation | |
| Field | Electrochemistry |
| Keywords | Elimination of particular currents; elimination polarography/voltammetry; DNA; adsorbed species |
| Description | Adsorptive stripping voltammetry has been extended by elimination voltammetry with the linear scan rate (EVLS). EVLS offers the elimination of selected currents from the linear scan voltammetry results. It can be achieved by an elimination function formed by a linear combination of total currents measured at different scan rates. The problem of substance adsorption influence on the elimination voltammetry results has been studied. Theoretical curves of an irreversible current with adsorbed substance being electroactive were calculated for three elimination functions. As a practical application, the adsorptive stripping voltammetry of thermally denatured DNA on a hanging mercury electrode has been developed. While the LSV signal of single-stranded DNA at low concentrations gives a slight hint of the cathodic peak, the EVLS provides quite a clear signal in the form of the peak-counterpeak. It is possible to determinate DNA at concentrations below of micrograms per milliliter. |
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