Kovalentní chromatografie

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Title in English Covalent chromatography
Authors

GLATZ Zdeněk

Year of publication 2000
Type Article in Periodical
Magazine / Source Chemické Listy
MU Faculty or unit

Faculty of Science

Citation
Field Biochemistry
Keywords covalent chromatography
Description Covalent chromatography is an important separation technique which is highly specific. In covalent chromatography proteins and other ligands bearing accessible thiol groups form a mixed disulphide with the gel. The proteins are thus covalently linked to the stationary phase from which they can be subsequently eluted by the addition of a reducing agent. The basic principles of the method are given together with the methods for the synthesis of gels. In addition, the paper describes several special application of covalent chromatography.
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