Immunohistochemical localization of laminin-1 in the acellular nerve grafts is associated with migrating Schwann cells which display corresponding integrin receptors

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Authors

DUBOVÝ Petr SVÍŽENSKÁ Ivana JANČÁLEK Radim KLUSÁKOVÁ Ilona HOUŠŤAVA Ladislav HANINEC Pavel

Year of publication 1999
Type Article in Periodical
Magazine / Source General Physiology and Biophysics
MU Faculty or unit

Faculty of Medicine

Citation
Field Neurology, neurosurgery, neurosciences
Keywords Acellular nerve graft; Integrins; Laminin-1; Schwann cell migration
Description The presence of laminin-1, collagen-IV, a6 and b1 integrin chains was detected by indirect immunohistochemistry using biotin/streptavidin/HRP or gold-conjugated secondary antibody at the light and electron microscope level, respectively. Cryo-treated segment of the peripheral stump without living Schwann cells (S-100-) did not display immunoreactivity for laminin-1 and integrin's chains, while the migrating Schwann cells in the marginal regions were immunostained for the antigens. Isolated acellular nerve segments protected from migration of Schwann cells (S-100-) exhibited laminin-l-, b1-, and a6- integrin chains immunoreactivities. Position of the basal lamina was verified by collagen-IV+ immunoreactivity. Results indicate that presence of the laminin in the peripheral nerve is related with living Schwann cells.
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