REACTIVATION OF A SILENT REGENERATIVE PROGRAM OF THE DORSAL ROOT GANGLIA NEURONS BY CONDITIONING LESION
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| Year of publication | 2016 |
| Type | Conference abstract |
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| Description | REACTIVATION OF A SILENT REGENERATIVE PROGRAM OF THE DORSAL ROOT GANGLIA NEURONS BY CONDITIONING LESION Dubový P., Svíženská-Hradilová I., Klusáková I., Brázda V. pdubovy@med.muni.cz Department of Anatomy, Faculty of Medicine, Masaryk University, Brno, Czech Republic The primary sensory neurons of dorsal root ganglia (DRG) are frequently used in vivo and in vitro models for reactivation of neuronal regenerative program. It is well documented that a peripheral nerve conditioning lesion triggers intrinsic regenerative program of the DRG neurons to enable regeneration not only peripheral, but also central branches of afferent axons present in the spinal cord. Reactivated regenerative program of the neurons is expressed by upregulation of regeneration-associated proteins like GAP-43. Based on our previous experimental results, we hypothesize that a peripheral conditioning lesion may trigger regenerative program also in the DRG neurons unrelated with injured nerve. In our present experiments, we analyzed upregulation of GAP-43 synthesis in the rat DRG neurons of C6-C8 spinal segments following the sciatic nerve injury. Quantitative RT-PCR and immunohistochemical analysis revealed significant bilateral elevation of GAP-43 mRNA and protein in the cervical DRG neurons after one week from unilateral sciatic nerve compression or transection. These results suggest that peripheral nerve conditioning lesion can trigger intrinsic regenerative program of the primary sensory neurons unrelated with injured nerve. This phenomenon reflects a systemic reaction of the nervous system structures alongside neural axis with signaling probably via the cerebrospinal fluid. Supported by grant 16-08508S of the Grant Agency of the Czech Republic. |
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