The 3D imaging of mesenchymal stem cells on porous scaffolds using high-contrasted x-ray computed nanotomography

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Authors	VOJTOVÁ Lucy ZIKMUND Tomáš PAVLIŇÁKOVÁ Veronika ŠALPLACHTA Jakub KALASOVÁ Dominika PROSECKÁ EVa BRTNÍKOVÁ Jana ŽÍDEK Jakub PAVLIŇÁK David KAISER Jozef
Year of publication	2019
Type	Article in Periodical
Magazine / Source	Journal of Microscopy
MU Faculty or unit	Faculty of Science
Citation
Web	Full Text
Doi	http://dx.doi.org/10.1111/jmi.12771
Keywords	biopolymeric scaffold;mesenchymal stem cells;SEM/EDX;tissue engineering; X-ray computed nanotomography
Description	This study presents an X-ray computed nanotomography (nano-CT) based, high-resolution imaging technique. Asample of highly porous collagen scaffold seeded with contrastedmesenchymalstemcells(MSC)was investigated by using lab-based nano-CT. The whole volume of the sample was analysed without its destruction. To evaluate the potential of nano-CT, a comparison measurement was done using a standard microscopy technique. Scanning electron microscopy (SEM) combined with energy dispersive X-ray analysis (EDX) established an extension and local accumulation of the contrasting agent – heavy metallic osmium tetroxide.
Related projects:	CEPLANT - Regionální VaV centrum pro nízkonákladové plazmové a nanotechnologické povrchové úpravy Rozvoj centra pro nízkonákladové plazmové a nanotechnologické povrchové úpravy CEITEC 2020