The adhesion of normal human dermal fibroblasts to the cyclopropylamine plasma polymers studied by holographic microscopy

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Authors

STRBKOVA L. MANAKHOV Anton ZAJÍČKOVÁ Lenka STOICA Adrian VESELY P. CHMELIK R.

Year of publication 2016
Type Article in Periodical
Magazine / Source SURFACE & COATINGS TECHNOLOGY
MU Faculty or unit

Central European Institute of Technology

Citation
Web http://www.sciencedirect.com/science/article/pii/S0257897215303728
Doi http://dx.doi.org/10.1016/j.surfcoat.2015.10.076
Field Solid matter physics and magnetism
Keywords Plasma polymer; Cyclopropylamine; Cell adhesion; Holographic microscopy; Quantitative phase imaging
Description The understanding of cell-surface interactions plays an important role for the biomaterials development and bioengineering. Although it is already known that amine groups increase the cell adhesion and proliferation, the influence of amine layers properties on cell viability is the subject of further investigation. In this work, amine-rich coatings were prepared by low pressure plasma polymerization of cyclopropylamine using radio frequency (RF) capacitively coupled discharge. Normal human dermal fibroblasts were chosen for the monitoring of biological response to the properties of amine layers. As a superior technique for the label-free monitoring of the cell-surface interaction, coherence-controlled holographic microscopy (CCHM) was exploited. CCHM enables quantitative phase imaging. From such images, valuable morphological parameters of cells directly related to the cell dry mass can be extracted. Based on those parameters, viability of cells cultivated on the plasma treated surfaces with different properties was studied and evaluated. According to the results, amine-rich films enhanced the conditions for the cell adhesion and proliferation. (C) 2015 The Authors. Published by Elsevier B.V.
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