Chromosome Preparation for Cytogenetic Analyses in Arabidopsis

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Authors	MANDÁKOVÁ Terezie LYSÁK Martin
Year of publication	2016
Type	Article in Periodical
Magazine / Source	Current protocols in plant biology
MU Faculty or unit	Central European Institute of Technology
Citation
Web	http://onlinelibrary.wiley.com/doi/10.1002/cppb.20009/abstract
Doi	http://dx.doi.org/10.1002/cppb.20009
Field	Genetics and molecular biology
Keywords	arabidopsis; Brassicaceae; mitotic and meiotic chromosomes; pachytene
Description	Despite its small nuclear genome and minute chromosomes, the model plant Arabidopsis thaliana has become a well established model in the field of plant cytogenetics. Since 2000, most cytogenetic and epigenetic approaches have been developed for this species and its congeners. In this chapter, we describe two step-by-step protocols for preparing chromosome preparations from root tip meristems and generative organ tissues of A. thaliana. Although both protocols have been optimized for A. thaliana, the detailed procedures are equally applicable to other members of the genus Arabidopsis as well as other crucifer species (Brassicaceae).
Related projects:	Evoluce a funkce komplexních genomů rostlin