The possibilities of studying human embryos and foetuses using micro-CT: a technical note

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Authors

VYMAZALOVÁ Kateřina VARGOVÁ Lenka ZIKMUND Tomáš KAISER Jozef

Year of publication 2017
Type Article in Periodical
Magazine / Source Anatomical Science International
MU Faculty or unit

Faculty of Medicine

Citation
Doi http://dx.doi.org/10.1007/s12565-016-0377-3
Field Other medical specializations
Keywords Micro-CT; Human embryo; Phosphotungstic acid; Staining
Description The purpose of the study was to discover a way to study the internal structure and evolution of human embryos noninvasively. The human embryo was stained with phosphotungstic acid solution (PTA) in ethanol (EPTA) and scanned using a micro computed tomography (micro-CT) scanner. Using appropriate software, a three-dimensional image of the embryo was created, which could be further exploited. The methodology described could be used for the non-destructive examination of the internal structure of the human embryo, and the resulting data can be used as a resource for medical students, gynaecologists, and paediatricians.
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