Mte1 interacts with Mph1 and promotes crossover recombination and telomere maintenance

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Authors

SILVA Sonia ALTMANNOVÁ Veronika LUKE-GLASER Sarah HENRIKSEN Peter GALLINA Irene YANG Xuejiao CHOUDHARY Chunaram LUKE Brian KREJČÍ Lumír LISBY Michael

Year of publication 2016
Type Article in Periodical
Magazine / Source Genes & Development
MU Faculty or unit

Faculty of Medicine

Citation
Doi http://dx.doi.org/10.1101/gad.276204.115
Field Genetics and molecular biology
Keywords homologous recombination; telomere maintenance; genome integrity; DNA repair; Mph1; Mte1
Description Mph1 is a member of the conserved FANCM family of DNA motor proteins that play key roles in genome maintenance processes underlying Fanconi anemia, a cancer predisposition syndrome in humans. Here, we identify Mte1 as a novel interactor of the Mph1 helicase in Saccharomyces cerevisiae. In vitro, Mte1 (Mph1-associated telomere maintenance protein 1) binds directly to DNA with a preference for branched molecules such as D loops and fork structures. In addition, Mte1 stimulates the helicase and fork regression activities of Mph1 while inhibiting the ability of Mph1 to dissociate recombination intermediates. Deletion of MTE1 reduces crossover recombination and suppresses the sensitivity of mph1 Delta mutant cells to replication stress. Mph1 and Mte1 interdependently colocalize at DNA damage-induced foci and dysfunctional telomeres, and MTE1 deletion results in elongated telomeres. Taken together, our data indicate that Mte1 plays a role in regulation of crossover recombination, response to replication stress, and telomere maintenance.
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