AFM for characterization of biomolecules, affinity complexes and cells

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Authors	SKLÁDAL Petr PŘIBYL Jan HORÁČKOVÁ Veronika PEŠL Martin BOUCHAL Pavel GEREG Patrik JAKUBEC Martin FARKA Zdeněk KOVÁŘ David
Year of publication	2015
Type	Conference abstract
MU Faculty or unit	Central European Institute of Technology
Citation
Description	The instrumentation and services available at the Core Facility of Nanobiotechnology, CEITEC Masaryk University, will be introduced. The research possibilities will be demonstrated on experiments with proteins, nucleic acids and their affinity complexes - interaction of ssDNA binding protein with oligonucleotides and immunoreaction between antibody and antigen. Properties of cellular membrane will be characterised in relation to the changes accompanying malignant transformation. Simultaneous recording of contractions and electric activity of cardiomyocytes will be presented using AFM cantilever with conductive tip functioning as nanomechanical transducer for cellular biosensor suitable for evaluation of fyziologically active compounds in real time.
Related projects:	CEITEC - Central European Institute of Technology