Wound Dressing based on Chitosan/hyaluronan/Nonwoven Fabrics: Preparation, Characterization and Medical Applications
| Authors | |
|---|---|
| Year of publication | 2016 |
| Type | Article in Periodical |
| Magazine / Source | International Journal of Biological Macromolecules |
| MU Faculty or unit | |
| Citation | |
| Web | odkaz na článek |
| Doi | http://dx.doi.org/10.1016/j.ijbiomac.2016.04.087 |
| Field | Plasma physics |
| Keywords | Hyaluronan;chitosan;nonwoven fabrics;cytotoxicity;healing properties |
| Description | Thin layers of chitosan (positively charged)/ sodium hyaluronate (negatively charged)/ nonwoven fabrics were constructed by polyelectrolyte multilayer pad-dry-cure technique. Pure chitosan (CS) was isolated from shrimp shell and immobilized onto nonwoven fabrics (NWFs) using citric acid (CTA) as cross linker and solvent agents through a pad-dry-cure method. The prepared thin layer of chitosan citrate/ nonwoven fabrics (CSCTA/NWFs) were consequently impregnated with hyaluronan (CSCTA/HA/NWFs) in the second path through a pad-dry-cure method. Chitosan/hyaluronan/nonwoven fabrics wound dressing was characterized by different tools such as FTIR-ATR, TGA and SEM. The antibacterial activity and the cytotoxicity of the dressing sheets were evaluated against Escherichia coli (E.coli) and Streptococcus aureus (St. aureus), mouse fibroblast (NIH-3T3) and keratinocytes (HaCaT) cell lines, respectively. The cell-fabrics 4 interaction was also investigated using fluorescence microscope, based on live/dead staining assay of 3T3 cells. The healing properties of the new wound dressing were evaluated and compared with the control sample. |
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