MODERN METHODS FOR DETERMINATION OF BINDING CONSTANTS: CAPILLARY ELECTROPHORESIS VS. ISOTHERMAL TITRATION CALORIMETRY
| Authors | |
|---|---|
| Year of publication | 2015 |
| Type | Conference abstract |
| MU Faculty or unit | |
| Citation | |
| Attached files | |
| Description | Strength of the binding between ligands (drugs) and proteins is commonly described by binding constant (Kb). These interactions have a significant effect on the biological activity, pharmacodynamics and pharmacokinetics properties of drugs. Kb can be determined by several methods. In this study capillary electrophoresis-frontal analysis (CE-FA) and isothermal titration calorimetry (ITC) are compared. Kb of systems diclofenac-HSA and lidocaine-HSA were measured by both methodologies. The results of CE-FA and ITC are comparable and can be used for investigation of other binding parameters. |
| Related projects: |