Nano scale derivatization followed by capillary electrophoresis with fluorescence detection
| Authors | |
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| Year of publication | 2014 |
| Type | Conference abstract |
| MU Faculty or unit | |
| Citation | |
| Description | Derivatization reaction allows modifying structure of originally non fluorescent compounds in order to detect original compounds by fluorescence detection. Such compounds could be amino acids, which play very important role in living organisms. Derivatization reagents are in general expensive and derivatization procedure is often time-consuming, laborious, and the products are instable. Narrow capillary can be also used as a reaction chamber of nano scale dimensions allowing automation of derivatization reaction minimizing manual steps, consumption of samples and reagents. Method development of on capillary derivatization reaction followed directly by capillary electrophoresis coupled with fluorescence detection of amino acids as well as optimization of separation conditions for analysis the reaction products are the subjects of this contribution. |
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