Determination of binding constants of tolbutamide/chlorpropamide with HSA
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| Year of publication | 2014 |
| Type | Conference abstract |
| MU Faculty or unit | |
| Citation | |
| Description | The binding constant (Kb) is commonly used to describe the strength of binding between ligand such as drug and protein. The strength of the interaction has a significant effect on biological activity of the drug. Tolbutamide and chlorpropamide are drugs in the sulfonylurea class used to treat type 2 diabetes mellitus. Various sulfonylureas (antidiabetic drugs) may induce hypoglycemia as a result of excesses in insulin production and release. Therefore, it is important to understand in detail the pharmacokinetics and pharmacodynamics of these drugs. The advantages of capillary electrophoresis for determination of binding constant are very low sample consumption, high resolution and also it does not require the highly purified samples, immobilization or labelling of any of the interacting species. The investigated interaction takes place in a solution, which can be simulated physiological conditions. The main objectives of this study were to determine the binding constants of the tolbutamide and chlorpropamide with HSA using capillary electrophoresis frontal analysis – in physiological conditions. |
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