Staphylococcus aureus in Czech cystic fibrosis patients – prospective study

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Authors

TKADLEC Jan MELTER Oto BUKÁČKOVÁ Eva FILA Libor PANTŮČEK Roman INDRÁKOVÁ Adéla BOTKA Tibor RŮŽIČKOVÁ Vladislava

Year of publication 2014
Type Article in Periodical
Magazine / Source Journal of Cystic Fibrosis
MU Faculty or unit

Faculty of Science

Citation
Web http://www.sciencedirect.com/science/article/pii/S1569199314602320
Doi http://dx.doi.org/10.1016/S1569-1993(14)60232-0
Field Genetics and molecular biology
Keywords Staphylococcus aureus; small colony variants; cystic fibrosis; enterotoxin genes
Description Altogether, 242 respiratory isolates of S. aureus from 105 patients with CF were collected in 2011–2012. The isolates were typed by PFGE and screened for susceptibility to antibiotics and presence of resistance and virulence genes. Small colony variants (SCV) were identified and tested for auxotrophy. Results: Total of 142/58 (isolates/patients) were resistant to MLSb antibiotics (Macrolides-Lincosamides-Streptogramin B), 25/13 were resistant to aminoglycosides and 6/5 were MRSA. MLSb resistance was associated with genes from erm family or msrA gene. Isolates resistant to aminoglycosides harboured aadC, aphA3 or aac-aphD gene. mecA gene was detected in all of 6 MRSA isolates. Most frequent virulence genes detected in the isolates (n = 242) were genes for enterotoxins seg (n = 137), sei (n = 128), sec (n = 38), sea (n = 19), sej (n = 15) and seh (n = 18). Other virulence genes were not found or found sporadically. Isolates with SCV phenotype (n = 19), all thymidine auxotrophs, were isolated in 9 patients. Isolates were separated in to the 39 different pulsotypes by PFGE with two dominant pulsotypes of 61 isolates from 27 patient (26%) and 28 isolates from 10 patients (12%) respectively.
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