Macrophage invasion into dorsal root ganglia in model of rheumatoid arthritis
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| Year of publication | 2012 |
| Type | Conference abstract |
| MU Faculty or unit | |
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| Description | Rheumatoid arthritis is a systemic, inflammatory autoimmune disorder that is manifested as a symmetric polyarthritis associated with swelling and pain in multiple joints. Generally, activated macrophages are involved in pathophysiology of inflammation. The aim of our present study was to compare a distribution of ED1+ activated macrophages int he lumbar dorsal root ganglia and changes of their amount in time. We used a rat model of unilateral antigen-induced arthritis (AIA). For induction of AIA, rats were immunized by an intra-articular injection of antigen methylated bovine serum albumin in saline emulsified with CFA into the knee joint. The knee joints of control rats were injected by saline and the naive rats were without AIA induction and injection of saline. All animals were left to survive for 4, 7, 14 and 21 days. Invasion of ED1+ macrophages was detected and quantified bilaterally in the lumbar dorsal root ganglia (L3-L5) using umage analysis system (NIS Elements). The data differences were evaluated by Mann-Whitney test using Statistica software. ED1+ macrophages were found bilaterally in lumbar L3-L5 dorsal root ganglia, especially in the L3 and L4. Four days after induction of AIA, we observed robust increasing of ED1+ macrophages in the ipsilateral lumbar ganglia. In contrast, contralateral ganglia have shown only a slight increase in the number of ED1+ macrophages. Seven and fourteen after immunization, all investigated dorsal root ganglia were infiltrated by approximately the same numbers of ED1+ macrophages. Twenty one days after AIA, i.e. during the chronic phase, we found no increased infiltration of ED1+ macrophages in ipsilateral dorsal root ganglia when compared to control and naive rats. However, in contralateral dorsal root ganglia we found approximately the same number of macrophages like seven or fourteen days after immunization. In summary, we observed bilateral invasion of ED1+ macrophages into dorsal root ganglia accompanying the acute, but not the chronic stage of AIA. The present study has shown that acute AIA is associated with a bilateral invasion of activated ED1+ macrophages into lumbar dorsal root ganglia involved in afferent innervation of the knee joint. |
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