"Click & Seed" Approach to the Biomimetic Modification of Material Surfaces

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Authors	PROKS Vladimír JAROŠ Josef POP-GEORGIEVSKI Ognen KUČKA Jan POPELKA Štěpán DVOŘÁK Petr HAMPL Aleš RYPÁČEK František
Year of publication	2012
Type	Article in Periodical
Magazine / Source	Macromolecular Bioscience
MU Faculty or unit	Faculty of Medicine
Citation
Doi	http://dx.doi.org/10.1002/mabi.201200095
Field	Genetics and molecular biology
Keywords	biomimetic modifications; click chemistry; peptide radiolabeling; polydopamine; poly(ethylene oxide)
Description	A simple, versatile, protein-repulsive, substrate-independent biomimetic surface modification is presented that is based on the creation of a PEO brush on a polydopamine anchoring layer and its capacity for selective follow-up modifications with various ligands using a coppercatalyzed alkyne-azide cycloaddition reaction. The desired surface concentration of peptide biomimetic ligands can be controlled by adjusting the peptide concentration in the reaction mixture, then measuring the activity of 125I-radiolabeled peptides that are immobilized on the substrates. The performance of the prepared substrates is tested in cell cultures with MEF cells and a human ECC line.
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