Hladiny miR-210 v nádorové tkáni a funkční efekty jejího utlumení na buňky renálního karcinomu

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Title in English The levels of miR-210 in tumor tissue and function of this miRNA in renal cell carcinoma
Authors

ILIEV Robert RÉDOVÁ Martina POPRACH Alexandr BEŠŠE Andrej KANTOROVÁ Kateřina NEKVINDOVÁ Jana LAKOMÝ Radek RADOVÁ Lenka SVOBODA Marek VYZULA Rostislav SLABÝ Ondřej

Year of publication 2012
Type Conference abstract
MU Faculty or unit

Central European Institute of Technology

Citation
Description Renal cell carcinoma (RCC) is the most frequent tumor disease of kidneys of adults. MicroRNAs (miRNAs) are short non-coding singlestranded RNAs that post-transcriptionally regulate gene expression. Using qRT-PCR TaqMan low density array (TLDA), global expression profiles of miRNAs in RCC and non-tumoral parenchym were analyzed. Data were normalized using RNU48 and RNU6B and subsequently analyzed in R using Bioconductor and hierarchical clustering. The results were further validated on independent set of samples. Finally, in vitro analyses were carried out using cell lines ACHN and CAKI-2 and effects of transfection of anti-miR-210 on cell viability, apoptosis, cell cycle, migration and invasivity were analyzed.
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