Hladiny miR-210 v nádorové tkáni a funkční efekty jejího utlumení na buňky renálního karcinomu
| Title in English | The levels of miR-210 in tumor tissue and function of this miRNA in renal cell carcinoma |
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| Authors | |
| Year of publication | 2012 |
| Type | Conference abstract |
| MU Faculty or unit | |
| Citation | |
| Description | Renal cell carcinoma (RCC) is the most frequent tumor disease of kidneys of adults. MicroRNAs (miRNAs) are short non-coding singlestranded RNAs that post-transcriptionally regulate gene expression. Using qRT-PCR TaqMan low density array (TLDA), global expression profiles of miRNAs in RCC and non-tumoral parenchym were analyzed. Data were normalized using RNU48 and RNU6B and subsequently analyzed in R using Bioconductor and hierarchical clustering. The results were further validated on independent set of samples. Finally, in vitro analyses were carried out using cell lines ACHN and CAKI-2 and effects of transfection of anti-miR-210 on cell viability, apoptosis, cell cycle, migration and invasivity were analyzed. |
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