Expression of hypothetical lectin from pisum sativum-comparison of two eukaryotic expression systems
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| Year of publication | 2012 |
| Type | Conference abstract |
| MU Faculty or unit | |
| Citation | |
| Description | We have identified a new putative lectin from Pisum sativum using SDS-PAGE and MS analysis of highly purified pea amine oxidase where it was present as an impurity. This study was aimed at the production of this protein in a eucaryotic expression system and its structure-function characterization. As production of the native protein was found very low, we have compared two yeast expression systems - Pichia pastoris and Saccharomyces cerevisiae. Advantages/disadvantages of both approaches will be discussed in detail. |
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